Description
ATP binding cassette sub-family member 2 (ABCG2) is a well-defined efflux transporter found in various tissues. The role of ABCG2 during early embryonic development, however, is not established. Previous work suggested an association exists between xenobiotics that regulate Abcg2 transcription and differentiation of mouse embryonic stem cells (mESC), a relationship potentially related to redox homeostasis. ABCG2 was found to serve protective role in mESC as a xenobiotic transporter. The role of ABCG2 in regulating redox status, however, was unclear. The hypothesis that ABCG2 plays a fundamental role during mESC differentiation or that regulation of the receptor by xenobiotics may be associated with altered mESC differentiation could not be supported.
This dataset is associated with the following publication:
Rosen, M., S. Jeffay, H. Nichols, M. Hoopes, and S. Hunter. ATP Binding Cassette Sub-Family Member 2 (ABCG2) And Xenobiotic Exposure During Early Mouse Embryonic Stem Cell Differentiation. Birth Defects Research. John Wiley & Sons, Inc., Hoboken, NJ, USA, 110(1): 35-47, (2017).
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| manuscript figure 1.marker genes across days.xlsx | XLS | manuscript%20figure%201.marker%20genes%20across%20days.xlsx | Open resource |
| manuscript figure 2.inhibitor exposure and normalized PhA FL on day 4.xlsx | XLS | manuscript%20figure%202.inhibitor%20exposure%20and%20normalized%20PhA%20FL%20on%20day%204.xlsx | Open resource |
| manuscript figure 3a.RedDot1 fluorescence on culture day 4 in mESC treated with topoisomerase inhibitors.xlsx | XLS | manuscript%20figure%203a.RedDot1%20fluorescence%20on%20culture%20day%204%20in%20mESC%20treated%20with%20topoisomerase%20inhibitors.xlsx | Open resource |
| manuscript figure 3b. Fold change in mESC on culture day 4 treated with topoisomerase inhibitors.docx | Doc | manuscript%20figure%203b.%20Fold%20change%20in%20mESC%20on%20culture%20day%204%20treated%20with%20topoisomerase%20inhibitors.docx | Open resource |
| manuscript figure 4a.PhA FL Day1 v Day4.xlsx | XLS | manuscript%20figure%204a.PhA%20FL%20Day1%20v%20Day4.xlsx | Open resource |
| manuscript figure 4b.Wes-simple western ABCG2 prot expression two reps.xlsx | XLS | manuscript%20figure%204b.Wes-simple%20western%20ABCG2%20prot%20expression%20two%20reps.xlsx | Open resource |
| manuscript figure 5a b.cell number and gene expression on culture day 4 n K0143 treated mESC.xlsx | XLS | manuscript%20figure%205a%20b.cell%20number%20and%20gene%20expression%20on%20culture%20day%204%20n%20K0143%20treated%20mESC.xlsx | Open resource |
| manuscript figure 6a.RedDot1 FL in ToxCast treated mESC on culture day9.xlsx | XLS | manuscript%20figure%206a.RedDot1%20FL%20in%20ToxCast%20treated%20mESC%20on%20culture%20day9.xlsx | Open resource |
| manuscript figure 6b. Fold change in ToxCast treated mESC on culture day 9.docx | Doc | manuscript%20figure%206b.%20Fold%20change%20in%20ToxCast%20treated%20mESC%20on%20culture%20day%209.docx | Open resource |
| manuscript figure 7a. RedDot1 Fl in TBHP and Paraquat treated mESC on culture day4.xlsx | XLS | manuscript%20figure%207a.%20RedDot1%20Fl%20in%20TBHP%20and%20Paraquat%20treated%20mESC%20on%20culture%20day4.xlsx | Open resource |
| manuscript figure 7b.Fold change in mESC on culture day 4 treated with tert-Butyl hydroperoxide and paraquat.docx | Doc | manuscript%20figure%207b.Fold%20change%20in%20mESC%20on%20culture%20day%204%20treated%20with%20tert-Butyl%20hydroperoxide%20and%20paraquat.docx | Open resource |