Description
This repository contains raw and intermediate data files as well as analysis code from the manuscript "Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services". In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| publication_set | Unknown | This folder contains code for two sets of data analyses (1) for NIST 16S amplicon sequencing and (2) for taxonomic profile comparisons in support of the evaluation of the analytical performance of DTC gut microbiome testing services conducted in R as well as input/output data files. a complete description can be found in the README file | Open resource |
| README | Text | Text file containing information/descriptions of folders and files included in this repository | Open resource |
| WMS_raw_reads | Unknown | The folder contains fastq files for data generated by NIST. a full description can be found in the README document | Open resource |
| publication set | Unknown | This folder contains code for two sets of data analyses (1) for NIST 16S amplicon sequencing and (2) for taxonomic profile comparisons in support of the evaluation of the analytical performance of DTC gut microbiome testing services conducted in R as well as input/output data files. a complete description can be found in the README file | Open resource |
| README | Text | READMEv2.txt | Open resource |
| Statistical Models | Unknown | This folder contains all files needed for the statistical models generated during data analysis | Open resource |
| WMS raw reads | Unknown | The folder contains fastq files for data generated by NIST. a full description can be found in the README document | Open resource |