Ambient Particulate Matter and Acrolein Co-Exposure Increases Myocardial Dyssynchrony in Mice via TRPA1

Description

We have examined the potential for interactive cardiovascular effects of repeated, intermittent co-exposure to concentrated ambient particulate matter (CAPs) and acrolein, and the potential role of transient receptor potential cation channel A1 (TRPA1), which we previously linked to air pollution-induced cardiac arrhythmogenesis. Chemical and source characteristics of collected particles was evaluated, as well as wind and weather patterns during exposure. Female B6129 mice and Trpa1-/- mice (n=6) were exposed to filtered air (FA), CAPs (46 µg/m3 of PM2.5 approximately 160 nm diameter), Acrolein (0.42 ppm) or CAPs+Acrolein for 3hrs/day, 2days/week, for 4 weeks. Cardiac strain data, heart function and dimensions, and transmitral blood flow were investigated with echocardiography (40 MHz) before exposures, 1 day after the first exposure, and 1 day after the final exposure. Several other biological endpoints were evaluated but the key findings from ultrasound echocardiography assessments were: elapsed time between peak strain in adjacent wall segments (i.e. myocardial strain delay), a measure of myocardial dyssynchrony, increased by ~5-fold in B6129 mice after the final exposure to CAPs+Acrolein when compared to strain delay in B6129 mice exposed to FA, CAPs, or Acrolein alone, and when compared to strain delay in Trpa1-/- mice exposed to CAPs+Acrolein. There were no changes after the first exposure in any group. This dataset is associated with the following publication: Thompson, L., L. Walsh, B. Martin, J. Mcgee, C. Wood, K. Kovalcik, P. Pancras, N. Coates, A. Ledbetter, D. Davies, W. Cascio, M. Higuchi, M. Hazari, and A. Farraj. Ambient Particulate Matter and Acrolein Co-Exposure Increases Myocardial Dyssynchrony in Mice: Evidence for TRPA1 Involvement. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 167(2): 559-572, (2019).

Resources

Name Format Description Link
53 CAPs%20Acrolein%20Source%20Apportionment.xlsx https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Source%20Apportionment.xlsx
53 CAPs%20Acrolein%20Chamber%20and%20Weather%20Values.xlsx https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Chamber%20and%20Weather%20Values.xlsx
53 CAPs%20Acrolein%20Elemental%20Analysis.xlsx https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Elemental%20Analysis.xlsx
53 CAPs%20Acrolein%20Biological%20Data.xlsx https://pasteur.epa.gov/uploads/10.23719/1411215/CAPs%20Acrolein%20Biological%20Data.xlsx

Tags

  • co-exposure
  • myocardial-dyssynchrony
  • ambient-air-quality
  • air-pollution-exposure
  • fine-particulate-matter
  • trpa1
  • myocardial-synchrony
  • heart-function
  • acrolein
  • echocardiography
  • multiple-pollutants

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