Adverse Outcome Pathway Networks I: Development and Applications
Description
In September, 2015, a water sample was collected downstream of a major metropolitan waste water treatment plant that discharges to the South Platte River, Colorado, USA. The grab sample, 1L, was collected just below the water surface, directly into a pre-cleaned, organic-free, amber glass bottle. The water sample was extracted by solid phase extraction using an Oasis-HLB glass catridge. Cartidges were conditioned sequentially using 5mL each of ethyl acetate, 50:50 methanol (MeOH):dichloromethane (DCM), MeOH, and water. The extract in DMSO was tested in the Attagene cis- and trans-FactorialTM assays (http://www.attagene.com/technology.php; Martin and others 2010; Romanov and others 2008). Data were analyzed using an established analysis pipeline for analyzing ToxCast™ high throughput screening data (Filer and others 2017). "Active hits" in the Attagene assay are included in the data table.
This dataset is associated with the following publication:
Knapen, D., M. Angrish, M. Fortin, I. Katsiadaki, M. Leonard, L. Mariotta-Casaluci, S. Munn, J. O'Brien, N. Pollesch, L.C. Smith, X. Zhang, and D. Villeneuve. Adverse outcome pathway networks I: Development and applications. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 37(6): 1723-1733, (2018).
Resources
| Name |
Format |
Description |
Link |
|
10 |
WG1-paper-1-supplementary-2018-02-08.docx |
https://pasteur.epa.gov/uploads/10.23719/1423303/WG1-paper-1-supplementary-2018-02-08.docx |
|
53 |
Attagene%20SP%202014-15_ScienceHub.xlsx |
https://pasteur.epa.gov/uploads/10.23719/1423303/Attagene%20SP%202014-15_ScienceHub.xlsx |
Tags
- screening-and-prioritization
- endocrine-disruption
- ecotoxicology
- adverse-outcome-pathway
- aquatic-ecosystems