Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Description

Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.

Resources

Name Format Description Link
21 Visit the original government dataset for complete information, documentation, and data access. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126239/

Tags

  • gene-expression
  • nih
  • normalization-methods
  • rt-pcr
  • housekeeping-genes

Topics

Categories