Description
This page hosts the AbsorbanceQ app which generates absorbance images from brightfield image captures. Absorbance microscopy can be used to make trypan blue cell viability measurements more quantitative and traceable by measuring the moles of trypan blue in each dead cell. AbsorbanceQ was written in MATLAB and can be downloaded as a .zip file. When unzipped, the program will be installed and can run as a standalone .exe file with no dependencies beyond the operating system. This website includes images that can be downloaded to validate that AbsorbanceQ is functioning properly. References: [1] Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021) Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy, in press. [2] Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021), Dataset for Absorbance Microscopy for Quantitative and Traceable Trypan Blue Cell Viability Measurement, National Institute of Standards and Technology, https://doi.org/10.18434/mds2-2347. [3] Zimmerman SM, Simon Jr CG, Babakhanova G (2021) AbsorbanceQ: An App for Generating Absorbance Images from Brightfield Images. J Res Natl Inst Stan 126:126039, https://doi.org/10.6028/jres.126.039.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| Zip File | ZIP | This is a zip file containing brightfield images of dead cells that can be used as input into the app in order to generate absorbance images. The images are [16 bit monochrome tif files]. There are 10 cell images I, one Imax image and one Imin image. | Open resource |
| SHA256 File for Cell_Images_16bit.zip | Text | Open resource | |
| Key_Code.docx | Doc | These are the key lines of functional code from the app that have been decoupled from the user interface. | Open resource |
| SHA256 File for Key_Code.docx | Text | Open resource | |
| License | Unknown | License | Open resource |
| MyAppInstaller_mcr.zip | ZIP | This is a zip file that contains a file called "MyAppInstaller_mcr.exe" that will install the AbsorbanceQ app on your computer. | Open resource |
| SHA256 File for MyAppInstaller_mcr.zip | Text | Open resource | |
| ReadMe file | Doc | ReadMe file that explains the contents of the website. | Open resource |
| SHA256 File for ReadMe file | Text | Open resource | |
| Source code | Doc | This is the Matlab source code for the AbsorbanceQ app. | Open resource |
| SHA256 File for Source code | Text | Open resource | |
| Synthetic_Images_16bit | ZIP | This is a .zip file containing synthetic images that can be used to validate that the app works properly using [16 bit monochrome .tif files] as input images. There are six synthetic neutral density filter images (I) (-0.1, 0.1, 0.5, 1.0, 1.5 and a striped image with four values), one synthetic Imax image and one synthetic Imin image. | Open resource |
| SHA256 File for Synthetic_Images_16bit | Text | Open resource | |
| Synthetic_Images_32bit | ZIP | This is a .zip file containing synthetic images that can be used to validate that the app works properly using [32 bit floating point monochrome .tif files] as input images. There are five synthetic neutral density filter images (I) (0.1, 0.5, 1.0, 1.5 and a striped image with four values), one synthetic Imax image and one synthetic Imin image. | Open resource |
| SHA256 File for Synthetic_Images_32bit | Text | Open resource | |
| Synthetic_Images_8bit | ZIP | This is a .zip file containing synthetic images that can be used to validate that the app works properly using [8 bit monochrome .tif files] as input images. There is a synthetic neutral density filter image (I) (0.5), one synthetic Imax image and one synthetic Imin image. | Open resource |
| SHA256 File for Synthetic_Images_8bit | Text | Open resource | |
| DOI Access for AbsorbanceQ App for Generating Absorbance Images from Brightfield Image Captures | Unknown | Open resource |