Description
In order to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential the following data have been produced:
- Physical-chemical characterization
- Interference test
- Liposome Cytotoxicity
- Effects of Liposomes on Complement Activation
- Inflammatory response
Out of eight differently functionalized liposomes selected as "case-studies", three passed the physical-chemical characterization ( in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| Complement activation | xlsx | ||
| Particle size distribution (PSD) and polydispersity index (PDI) of liposomes performed by Dynamic Light Scattering (DLS) | xlsx | ||
| Inflammatory response (Origin project) | ZIP | ||
| UV-Vis spectra of liposomes | xlsx | ||
| Liposome interference with the chromogenic LAL assay’ readout of pNA at 405 nm | xlsx | ||
| Liposome interference with the chromogenic LAL assay’ readout of pNA+ azo-dye at 540 nm | xlsx | ||
| Liposome Cytotoxicity (GraphPad Prism) | ZIP | ||
| Stokes radius of four selected liposomes (F10103, F10102, F20103A and F20104A) measured by analytical ultracentrifuge (AUC) | xlsx | ||
| Hydrodynamic diameter measured by applying the Flow-field-flow fractionation (AF4) system | xlsx |