Characterization of Liposome-based Nanomaterials
Description
In order to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential the following data have been produced:
- Physical-chemical characterization
- Interference test
- Liposome Cytotoxicity
- Effects of Liposomes on Complement Activation
- Inflammatory response
Out of eight differently functionalized liposomes selected as "case-studies", three passed the physical-chemical characterization ( in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not.
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Tags
- safety-assessment
- nanomaterial
- cytokines
- innterleukin
- inflammation
- endotoxin
- limulus-amoebocyte-lisate-(lal)
- nanomedicine
- liposome
- physicochemical-characterization
- particle-size-distribution