Description

The Eastern Shore Islands Area of Interest (AOI) is a large coastal AOI in the Maritimes Region spanning 2089 km2 and extending along 100 km of coastline. While much of the data that led to the area's designation as an AOI was collected in the nearshore coastal areas (i.e., seagrass and kelp distributions, coastal bird nesting sites, herring spawning areas), there is less information available on the fish and invertebrate community structure in offshore areas of the AOI. Current offshore sampling effort is primarily focused in the Summer multi-species RV survey which covers the Scotian Shelf but does not sample within the depth range covered by the AOI (~ <100m depth). Environmental DNA (eDNA) offers a promising approach for biodiversity monitoring that has been gaining increased attention in the marine realm. Through sampling relatively small volumes of water (~1-5 L), eDNA can capture of millions of fragments of eDNA on filters that can then be sequenced to identify the species composition of an area. eDNA metabarcoding is based on the foundations of DNA barcoding, where species are identified solely by their unique DNA sequences. eDNA metabarcoding relies on a comprehensive reference library of gene sequences from known species to rapidly identify which species are captured in every water sample. This method is relatively simple, efficient (with the ability to characterize both fish and invertebrate diversity), and non-invasive, meaning there is no disruption of sensitive benthic habitats or need to dissect tissue samples from captured fish and invertebrates. eDNA has been shown to be comparable to other biodiversity censusing techniques and has the potential to rapidly conduct biodiversity surveys over a relatively large area, such as the Eastern Shore Islands AOI. Our eDNA sampling in the Eastern Shore Islands targets fish and invertebrates using multiple genetic markers (e.g., 12S and COI) to obtain baseline information on these communities across transects spanning from inshore to offshore in the AOI. Over time, we will investigate changes in species richness and community composition using annual eDNA surveys as an ongoing monitoring tool for this coastal region. Cite this data as: Jeffery, N.W. Environmental DNA Monitoring in the Eastern Shore Islands Area of Interest - Offshore. Published: April 2026. Coastal Ecosystems Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth NS.

Resources

Name	Format	Description	Link
Metabarcoding results from the12S marker	ZIP		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/12S_Fish_MetabarcodingResults.zip
12S raw sequences	ZIP		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/12S_Raw_FASTQ_2022.zip
Metabarcoding results from the 16S marker	ZIP		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/16S_Fish_MetabarcodingResults.zip
16S raw sequences	ZIP		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/16S_Raw_FASTQ_2022.zip
Metabarcoding results from the COI marker	ZIP		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/COI_Eukaryotes_MetabarcodingResults.zip
COI raw sequences	ZIP		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/COI_Raw_FASTQ_2022.zip
Data dictionary	CSV		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/Data_dictionary.csv
Sampling station metadata from 2022	Unknown		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/ESI_eDNA_Offshore_2022.zip
Sampling station metadata from 2022	CSV		https://api-proxy.edh-cde.dfo-mpo.gc.ca/catalogue/records/0fe7b19d-864c-47fa-b533-c38a09519f9d/attachments/GOTeDNA_ESIoffshore_Sampling_Metadata_2022.csv
Environmental DNA Monitoring in the Eastern Shore Islands Area of Interest - Offshore	Ersi REST		https://egisp.dfo-mpo.gc.ca/arcgis/rest/services/open_data_donnees_ouvertes/environmental_dna_monitoring_esi_aoi/MapServer
Environmental DNA Monitoring in the Eastern Shore Islands Area of Interest - Offshore	Ersi REST		https://egisp.dfo-mpo.gc.ca/arcgis/rest/services/open_data_donnees_ouvertes/environmental_dna_monitoring_esi_aoi/MapServer

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